Dietary Stearic Acid Leads to a Reduction of Visceral Adipose Tissue in Athymic Nude Mice
نویسندگان
چکیده
Stearic acid (C18:0) is a long chain dietary saturated fatty acid that has been shown to reduce metastatic tumor burden. Based on preliminary observations and the growing evidence that visceral fat is related to metastasis and decreased survival, we hypothesized that dietary stearic acid may reduce visceral fat. Athymic nude mice, which are used in models of human breast cancer metastasis, were fed a stearic acid, linoleic acid (safflower oil), or oleic acid (corn oil) enriched diet or a low fat diet ad libitum. Total body weight did not differ significantly between dietary groups over the course of the experiment. However visceral fat was reduced by ∼70% in the stearic acid fed group compared to other diets. In contrast total body fat was only slightly reduced in the stearic acid diet fed mice when measured by dual-energy x-ray absorptiometry and quantitative magnetic resonance. Lean body mass was increased in the stearic acid fed group compared to all other groups by dual-energy x-ray absorptiometry. Dietary stearic acid significantly reduced serum glucose compared to all other diets and increased monocyte chemotactic protein-1 (MCP-1) compared to the low fat control. The low fat control diet had increased serum leptin compared to all other diets. To investigate possible mechanisms whereby stearic acid reduced visceral fat we used 3T3L1 fibroblasts/preadipocytes. Stearic acid had no direct effects on the process of differentiation or on the viability of mature adipocytes. However, unlike oleic acid and linoleic acid, stearic acid caused increased apoptosis (programmed cell death) and cytotoxicity in preadipocytes. The apoptosis was, at least in part, due to increased caspase-3 activity and was associated with decreased cellular inhibitor of apoptosis protein-2 (cIAP2) and increased Bax gene expression. In conclusion, dietary stearic acid leads to dramatically reduced visceral fat likely by causing the apoptosis of preadipocytes.
منابع مشابه
The Association of Omentin Gene Expression in Visceral and Subcutaneous Adipose Tissues with Plasma Fatty Acids Profile and Dietary Fatty Acids
Introduction: Omentin, an adipokine, with anti-inflammatory effects reduces insulin resistance, and can hence, play an important role in prevention of cardiovascular disease and diabetes. The present study aimed to investigate the association of plasma and dietary fatty acids with gene expression of omentin in visceral and subcutaneous adipose tissues. Materials and Methods: Visceral and subcut...
متن کاملComparison of Adipose tissue fatty acide in CAD and non-CAD patients
since long term dietary fatty acide intake is reflected in the fatty acide composition of Adipose tissue, this study was aimed to compare the adippose tissue fatty acide profile of angiography documented CAD (test)and non-CAD(control) patients.the first group were 25 patients who underwent coronary artery bypass graft surgery in open heart surgery section of Shahid Beheshti hospital of Kerman...
متن کاملAssociation of some dietary intakes, anthropometric measurements and insulin resistance with the relative P53 gene expression in visceral and subcutaneous adipose tissue in obese, and non-obese subjects
Background and Objectives: The P53 is one of the genes involved in weight management. This study investigated associations of dietary intakes, anthropometric measurements and insulin resistance with relative P53 gene expressions. Materials & Methods: Visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) were collected from 151 individuals, aging nearly 40 years, who underwent el...
متن کاملInjectable Estradiol Valerate, as a Substitute for Estradiol Pellets in Breast Cancer Animal Model
The ability to maintain and study human tissues in in-vivo environment has proved to be a valuable tool in breast cancer research for several decades. The most widely tissues have been xenografts established human breast cancer cell lines into athymic nude mice. The aim of this study was to provide a new accurate and affordable method for the establishment of breast cancer xenograftin nude mice...
متن کاملThe effect of interval and continuous training on the content of perilipin 1, ATGL and CGI-58 in visceral adipose tissue of obese male rats
Background and Aim: Obesity is an excessive accumulation of fat and its storage due to an imbalance between energy intake and energy consumption. High-fat diets, through inhibition of lipolysis enzymes can lead to increase in obesity and also many metabolic diseases. While exercise can activate these enzymes and lead to a change in the amount of visceral adipose tissue and reduce obesity. The p...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 9 شماره
صفحات -
تاریخ انتشار 2014